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REVIEW: Design of Pyridoxal 5´-Phosphate Dependent Catalytic Antibodies

R. M. Khomutov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia; fax: (095) 135-1405; E-mail: khomutov@genome.eimb.relarn.ru

Received May 7, 2002
Modern approaches for developing antibodies with coenzyme-dependent activities are discussed for pyridoxal 5´-phosphate dependent transformation of amino acid as an example. A new type of antigens analogous to enzyme-substrate compounds is suggested for the production of such antibodies. Approaches for the development of pyridoxal antiidiotypic antibody using analogs of coenzyme-substrate compounds and corresponding apoenzyme complexes are reviewed.
KEY WORDS: transition state analogs, B6 enzymes, haptens, enzyme transition state analogs, coenzyme dependent catalytic antibody