2Institute of Physico-Chemical Medicine, Ministry of Health of Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119992 Russia; E-mail: email@example.com
* To whom correspondence should be addressed.
Received April 17, 2002; Revision received May 25, 2002
This review highlights modern technologies employed in proteomics. Methods of sample preparations are discussed with special emphasis on the requirements for preparation of biological material, which may seriously influence the results of proteomic studies. Methods of solubilization, electrophoresis, chromatographic protein separation, and visualization of protein spots in gels are described. Modern methods of mass spectrometry used in proteomic studies include combination of protein chips with mass spectrometry. The review also describes approaches of functional proteomics, i.e., interactomics, and also bioinformatic resources used in proteomics for image analysis of 2D-gel-electrophoresis and for identification of protein sequences by mass spectra.
KEY WORDS: proteomics, biotechnology, electrophoresis, chromatography, protein chip