Pentaspan Membrane Glycoprotein, Prominin-1, Is Involved in Glucose Metabolism and Cytoskeleton Alteration

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Chang Yang¹, Yanli Yang¹, Nishith Gupta², Xiaojun Liu¹, Aibin He¹, Lizhong Liu¹, Jin Zuo¹, Yongsheng Chang^1*, and Fude Fang^1*

¹National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, P. R. China; fax: +86-10-6525-3005; E-mail: fangfd@vip.sina.com; changyongsheng@yahoo.com

²Institute of Molecular Parasitology, Humboldt University, Berlin-10115, Germany

^* To whom correspondence should be addressed.

Received March 16, 2007; Revision received April 25, 2007

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This study constitutes the first report revealing the participation of prominin-1 in glucose metabolism and cytoskeleton alteration. Upon stimulation with high glucose, the expression of prominin-1 was up-regulated with concomitant down-regulation of its phosphorylation. Prominin-1 mutated at its phosphorylation site inflicted a global change in the expression of several genes associated with glucose metabolism in L6 myotube cells. Further, the over-expression of prominin-1 promoted glucose uptake in these cells. Prominin-1 undergoes sustained repression of its expression during confluence and differentiation of L6 myotube cells. The expression of prominin-1 in the MDCK cell modulated cell morphology and promoted cellular migration. These data imply that prominin-1 is involved in glucose metabolism and may regulate cellular glucose through its effect on cytoskeleton.

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KEY WORDS: prominin-1, CD133/AC133, glucose, cytoskeleton

DOI: 10.1134/S000629790708007X

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