International Biotechnological Center, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-5022
Received March 1, 2008; Revision received April 30, 2008
Steady-state kinetics of compulsory-ordered single-substrate irreversible and reversible enzyme reactions with two, three, and arbitrary number of intermediates were observed. Necessary and sufficient conditions for application of the quasi-equilibrium assumption and restrictions of this assumption were found in cases of two and three intermediates in the equilibrium segment. For all cases, accuracy of the quasi-equilibrium assumption was evaluated.
KEY WORDS: enzyme kinetics, quasi-equilibrium assumption, rapid equilibrium, theory, steady-state kinetics, Cha method, accuracy of assumption