Extracellular Chitinases of Mutant Superproducing Strain Serratia marcescens M-1

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A. B. Duzhak^1*, Z. I. Panfilova¹, T. G. Duzhak^1,2, and E. A. Vasyunina¹

¹Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, pr. Akademika Lavrentieva 10, 630090 Novosibirsk, Russia; fax: (383) 333-1278; E-mail: abduzhak@mail.ru

²University of Kansas, 2121 Simons Drive, Lawrence, KS 66047 USA; fax: 785-8643787; E-mail: tgduzhak@mail.ru

^* To whom correspondence should be addressed.

Received February 7, 2008; Revision received February 18, 2008

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Four extracellular proteins with chitinase activity capable of binding chitin substrates have been revealed in the culture liquid of chitinase superproducing mutant strain M-1 of Serratia marcescens. Proteins were analyzed by SDS-PAGE and MALDI-TOF mass spectrometry. Based on the data obtained, the proteins were identified as typical chitinases of S. marcescens: ChiA, ChiB, ChiC, and CBP21.

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KEY WORDS: chitinase, colloidal chitin, Serratia marcescens, mass spectrometry

DOI: 10.1134/S0006297909020126

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