Identification of Proteins Specifically Interacting with YB-1 mRNA 3′ UTR and the Effect of hnRNP Q on YB-1 mRNA Translation

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D. N. Lyabin, L. F. Nigmatullina, A. N. Doronin, I. A. Eliseeva, and L. P. Ovchinnikov^*

Institute of Protein Research, Russian Academy of Sciences, ul. Institutskaya 4, 142290 Pushchino, Moscow Region, Russia; fax: +7 (495) 514-0218; E-mail: ovchinn@vega.protres.ru

^* To whom correspondence should be addressed.

Received January 28, 2013; Revision received February 27, 2013

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In this study, proteins specifically interacting with the 3′ untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3′ UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator – YB-1 itself.

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KEY WORDS: protein YB-1, protein hnRNP Q, translation regulation, 3′ untranslated region of mRNA

DOI: 10.1134/S0006297913060102

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