* To whom correspondence should be addressed.
Received January 28, 2013; Revision received February 27, 2013
In this study, proteins specifically interacting with the 3′ untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3′ UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator – YB-1 itself.
KEY WORDS: protein YB-1, protein hnRNP Q, translation regulation, 3′ untranslated region of mRNA