* To whom correspondence should be addressed.
Received February 21, 2013; Revision received February 26, 2013
We have found that extracellular phosphomannan is the main phosphate reserve in the yeast Kuraishia capsulata, in contrast to other yeast species effectively absorbing Pi. Under nitrogen starvation, K. capsulata absorbed essentially all Pi from the medium containing 240 mM glucose, 2.5 mM MgSO4, and 11 mM KH2PO4. Inorganic polyphosphate level in the cells was about 14% of the Pi absorbed. Most of the Pi (~60%) was found in the fraction of extracellular phosphomannan that can be used as a carbon and phosphorus source by this yeast in deficient media.
KEY WORDS: inorganic polyphosphate, phosphomannan, phosphate accumulation, nitrogen deficit, yeast, Kuraishia capsulata