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Development of Approaches to Creation of Experimental Test System for Evaluation of Antigenic Activity of Synthetic Oligosaccharide Ligands Related to Fragments of the Streptococcus pneumoniae Type 14 Capsular Polysaccharide

E. A. Kurbatova1*, D. S. Vorobiov1, I. B. Semenova1, E. V. Sukhova2, D. V. Yashunsky2,3, Y. E. Tsvetkov2, and N. E. Nifantiev2

1Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Malyi Kazennyi Pereulok 5a, 105064 Moscow, Russia; fax: (495) 917-4900; E-mail: kurbatova6162@yandex.ru

2Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia; fax: (499) 135-8784; E-mail: nen@ioc.ac.ru

3Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10/8, 119121 Moscow, Russia

* To whom correspondence should be addressed.

Received December 22, 2012; Revision received January 26, 2013
A conjugate of a synthetic hexasaccharide fragment of the Streptococcus pneumoniae type 14 capsular polysaccharide with bovine serum albumin (BSA) has been prepared. The antigenic activity and specificity of this conjugate are comparable with those of natural antigens of S. pneumoniae type 14. The data suggest that the resulting synthetic conjugate can be used as a coating antigen in an experimental test system (based on enzyme immunoassay) for evaluating the antigenic activity and specificity of synthetic oligosaccharide ligands and for testing specimens of natural capsular polysaccharides and immune sera.
KEY WORDS: Streptococcus pneumoniae type 14, capsular polysaccharide, synthetic oligosaccharide fragment, oligosaccharide–BSA conjugate, antigenic activity

DOI: 10.1134/S0006297913070122