Level of Blood Cell-Free Circulating Mitochondrial DNA as a Novel Biomarker of Acute Myocardial Ischemia

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N. P. Sudakov^1,2,3, T. P. Popkova², A. I. Katyshev⁴, O. A. Goldberg¹, S. B. Nikiforov¹, B. G. Pushkarev¹, I. V. Klimenkov^2,5, S. A. Lepekhova^1,3, K. A. Apartsin^1,3, G. A. Nevinsky⁶, and Yu. M. Konstantinov^2,4*

¹Irkutsk Surgery and Traumatology Research Center, 664003 Irkutsk, Russia

²Irkutsk State University, 664003 Irkutsk, Russia

³Irkutsk Research Center, Siberian Branch of the Russian Academy of Sciences, 663033 Irkutsk, Russia

⁴Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia; E-mail: yukon@sifibr.irk.ru

⁵Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia

⁶Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia

^* To whom correspondence should be addressed.

Received July 27, 2015; Revision received August 6, 2015

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Changes in the level of blood cell-free circulating mitochondrial DNA were examined during experimental adrenaline-induced myocardial injury in rats. The amount of mitochondrial DNA in the blood was significantly elevated at 48 and 72 h after subcutaneous injection of adrenaline solution, and it was accompanied by development of multiple small-focal myocardial ischemia. This suggests that the measured level of blood cell-free circulating mitochondrial DNA might be used as a biomarker of acute myocardial ischemia.

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KEY WORDS: acute ischemic myocardial injury, cell-free circulating mtDNA, cytolysis biomarkers, real-time PCR

DOI: 10.1134/S000629791510020X

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