REVIEW: Apoptosis in Cryopreserved Eukaryotic Cells

---

M. A. Savitskaya^* and G. E. Onishchenko

Lomonosov Moscow State University, Biological Faculty, 119991 Moscow, Russia; fax: +7 (495) 939-4309; E-mail: nakomis@mail.ru

^* To whom correspondence should be addressed.

Received July 6, 2015; Revision received December 2, 2015

---

This review considers apoptosis mechanisms that have been revealed in cryopreserved cells and which can be controlled using different chemical agents, thereby improving the viability of cells after their return to normal conditions. The role of oxidative stress as of the most significant damaging factor is discussed, as well as the reasonability of including antioxidants into cryopreservation/thawing protocols as independent agents or in combination with other compounds.

---

KEY WORDS: apoptosis, cryopreservation, oxidative stress, autophagy

DOI: 10.1134/S0006297916050023

---