Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis

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Hailiang Ma¹, Yuanben Lu¹, Dewen Zhu¹, Zhenhua Jiang¹, FanZhi Zhang², Jun Peng^3,a*, and Li Wang^1,b*

¹Department of Cardiovascular Medicine, Shaoxing Central Hospital, Shaoxing City, Zhejiang Province, 312000, China

²Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, China

³Department of Cardiovascular Medicine, First People’s Hospital of Xiaoshan District, Hangzhou 311200, China

Received May 22, 2023; Revised December 23, 2023; Accepted April 19, 2024

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Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. We explored the protective effect of GP on human cardiomyocytes (HCMs) via the circ_0010729/miR-370-3p/RUNX1 axis. Overexpression of circ_0010729 abolished the effects of GP on HMC, such as suppression of apoptosis and increase in cell viability and proliferation. Overexpression of miR-370-3p reversed the effect of circ_0010729 overexpression, resulting in the stimulation of HMC viability and proliferation and inhibition of apoptosis. The knockdown of miR-370-3p suppressed the effects of GP in HCMs. RUNX1 silencing counteracted the effect of miR-370-3p knockdown and maintained GP-induced suppression of apoptosis and stimulation of HMC viability and proliferation. The levels of RUNX1 mRNA and protein were reduced in cells expressing miR-370-3p. In conclusion, this study confirmed that GP alleviated the I/R injury of myocardial cell via the circ_0010729/miR-370-3p/RUNX1 axis.

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KEY WORDS: gypenoside A, ischemia/reperfusion injury, circ_0010729, miR-370-3p, RUNX1, oxygen and glucose deprivation/reoxygenation

DOI: 10.1134/S000629792405016X

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