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Biochemistry (Moscow). Papers in Press. Published on November 6, 2017 as Manuscript BM17-211.

Interaction between RAD51 and MCM Complex Is Essential for RAD51 Foci Forming in Colon Cancer HCT116 Cells

Jun Huang, Hong-Liang Luo, Hua Pan, Cheng Qiu, Teng-Fei Hao, and Zheng-Ming Zhu*

Second Affiliated Hospital of Nanchang University, Department of Gastrointestinal Surgery, Nanchang 330006, China; E-mail: zhuzhengming7088@126.com

*To whom correspondence should be addressed.

Received May 8, 2017; Revision received October 25, 2017
Colon cancer remains one of the most common digestive system malignancies in the World. This study investigated the possible interaction between RAD51 and minichromosome maintenance proteins (MCMs) in HCT116 cells, which can serve as a model system for forming colon cancer foci. The interaction between RAD51 and MCMs was detected by mass spectrometry. Silenced MCM vectors were transfected into HTC116 cells. The expressions of RAD51 and MCMs were detected using Western blotting. Foci forming and chromatin fraction of RAD51 in HCT116 cells were also analyzed. The results showed that RAD51 directly interacted with MCM2, MCM3, MCM5, and MCM6 in colon cancer HTC116 cells. Suppression of MCM2 or MCM6 by shRNA decreased the chromatin localization of RAD51 in HTC116 cells. Moreover, silenced MCM2 or MCM6 decreased the foci forming of RAD51 in HTC116 cells. Our study suggests that the interaction between MCMs and RAD51 is essential for the chromatin localization and foci forming of RAD51 in HCT116 cell DNA damage recovery, and it may be a theoretical basis for analysis of RAD51 in tumor samples of colon cancer patients.
KEY WORDS: colon cancer, HTC116 cells, RAD51, MCM complex, homologous recombination

DOI: 10.1134/S0006297918010091