2Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119334 Moscow, Russia; E-mail: email@example.com
* To whom correspondence should be addressed.
Received May 21, 2014
We found that some morphological properties of the pineal gland and submandibular salivary gland of mice are significantly distinct at the new and full moon. We suppose that the differences are initiated by the displacements of the electron-dense concretions in the secretory vesicles of pinealocytes. This presumably occurs under the influence of the gravitational field, which periodically changes during different phases of the moon. It seems that the pinealocyte is both an endocrine and gravisensory cell. A periodic secretion of the pineal gland probably stimulates, in a lunaphasic mode, the neuroendocrine system that, in turn, periodically exerts influence on different organs of the body. The observed effect probably serves, within the lifelong clock of a brain, to control development and aging in time.
KEY WORDS: pinealocytes, electron-dense concretions in secretory vesicles, lunaphasic changes of cell morphology
The so-called light pinealocytes (LP) and dark pinealocytes (DP) are mosaically located in the pineal gland of the mouse. The cytoplasm of LP and DP contains vesicles that are presumably involved in the secretory activity of this endocrine organ as key participants of the lunaphasic response of the body. Based on this assumption, we searched and found morphological distinctions in some structures of pineal and submandibular salivary glands during the opposite phases of the lunar cycle. These distinctions are apparently the body’s response to the corresponding phases of the natural celestial satellite of the Earth.
MATERIALS AND METHODS
The study was performed in accordance with relevant institutional regulations on 12-week-old male mice (stock CD1) weighing 25 to 30 g (n = 22). The glands were removed at 11 a.m. in the different phases of the lunar cycle, after a 24-h food deprivation of animals. Salivary glands were fixed in 10% neutral formalin, then dehydrated and embedded in paraffin. The sections were stained with hematoxylin and eosin. The pineal glands were fixed by immersion in a mixture of 4% paraformaldehyde and 2.5% glutaraldehyde in a 0.1 M cacodylate buffer (pH 7.4), then post-fixed in a 1% solution of osmium tetroxide, dehydrated, and embedded in the Epon-Araldite resin mixture. The sections were prepared on a Leica EM UC 7 ultramicrotome (Austria). Semi-thin sections were stained with azure II. The light-microscopic study was performed on the Primo Star microscope (Carl Zeiss, Germany) with the G-10 digital camera (Canon, Japan) and Axio Vision 4.8.2 software (Carl Zeiss). The number of profiles of pinealocytes and large cytoplasmic vesicles with osmiophilic inclusions was counted in 10 fields of each section of the pineal gland having area of 0.011 mm2 (objective ×100, ocular ×10) followed by the measurement of their size, the area of nuclei, and the diameter of pinealocyte nucleoli. In a cross-section of the submandibular salivary glands, we measured the lumen area of the granular segments, the area of the nucleus, and the nuclear-cytoplasmic ratio of epitheliocytes. Ultra-thin sections of the pineal glands contrasted with uranyl acetate and lead citrate were examined on a Philips CM 12 electron microscope (Netherlands) with Megaview G 2 digital camera (Olympus, Germany). The data from the Excel 7.0 general matrix (Microsoft, USA) were processed by the methods of variation statistics using the Statistics for Windows v.6.0 software package. The arithmetic mean value of the index and the error of the mean were calculated. The significance of differences was judged by Student’s t-test. The differences were considered significant at P < 0.05.
No significant differences between the total number of pinealocytes in the pineal gland of mice at the new and full moon were revealed. Also, the average area of the nucleus and the average diameter of the nucleolus of these cells were practically independent of the phases of the moon (table). The phases of the moon did not affect the distribution of the two types of pinealocytes, namely light (LP) and dark (DP) pinealocytes, among the zones of the organ, namely in the deep and surface zones of the pineal gland (Fig. 1).
Average number of pinealocytes and their karyometric indices for the
different phases of the moon (M ± m)
Fig. 1. Pinealocytes distribution by their types and by the zones of the pineal gland in the new moon and the full moon.
However, morphological differences for LP and DP between the new moon and the full moon proved to be significant at the intracellular level. Not only the total number of vesicles with osmiophilic inclusions increases (by more than half) in the full moon as compared with the new moon, but their distribution between different types of cells and within cells also proved to be uneven. Only one third of all vesicles were found in the DP in the new moon, whereas in the full moon there are almost half of them (Fig. 2). Vesicles with osmiophilic inclusions are basically found in perikaryons. Less than a quarter of all the vesicles are found in the processes of pinealocytes at the new moon, whereas at the full moon there are more than one third of them there, which indirectly indicates the shift of the vesicles, closer to the full moon, in the direction of the pinealocytic processes (Fig. 3).
Fig. 2. Distribution of vesicles with osmiophilic inclusions in light and dark cells at the different phases of the moon cycle. The proportion of vesicles of light and dark cells in the specified phase of the moon cycle is shown on the columns.
Fig. 3. Distribution of vesicles with osmiophilic inclusions in the processes and perikaryones of pinealocytes at the different phases of the moon cycle. The proportion of vesicles in the processes and perikaryones in the specified phase of the moon cycle is shown on the columns.
Figures 2-5 show large differences in the location and size of the vesicles with osmiophilic inclusions in the opposite phases of the lunar cycle, specifically, in the new moon and in the full moon. These differences can be seen when comparing the number of vesicles in the LP and DP in the surface and deep zones of the pineal gland, as well as when comparing representation of the vesicles in perikaryons and in the processes of pinealocytes. The number of vesicles in the processes of LP and DP of the deep zone of the gland increased 5.1 to 7.7 times in the full moon as compared with that in the new moon.
Fig. 4. Dependence of the number of vesicles in the pinealocytic processes within the deep zone of the pineal gland on the specified phase of the moon.
Fig. 5. Dependence of the diameter of the vesicles in the perikaryones of pinealocytes within the deep zone of the pineal gland on the specified phase of the moon.
In the submandibular salivary glands of mice, the phasic changes of morphology are also associated with the lunar periodicity, and they were observed in the granular compartments of striated excretory ducts, which are involved in the secretory function. At the new moon as compared with the full moon, these salivary glands contain an increased number of large secretory granules and a reduced lumen area of granular compartments in cross section (Fig. 6); also in the new moon, the area of the nuclei in epitheliocytes and their nuclear-cytoplasmic ratio are reduced.
As concerns the origin of the vesicles, the following point should be noted. In the new moon, hyperplasia of dictyosomes and increase in the size of the Golgi apparatus area were observed in the LP with a large nucleus and nucleoli of the reticular type. In the full moon, dictyosomes are located near the nucleus, sometimes directly in the folds of karyolemma, dictyosomes break with the formation of vesicles, and the number of dictyosomes decreases. The photos shown in Fig. 7 depict the region of the Golgi complex in pinealocytes in the different phases of the moon. Vesicles represent themselves as containers, supposedly carrying in them not only dense inclusions, but also secretory factors (photos in Fig. 8).
Fig. 7. Photos of the Golgi complex in pinealocytes: a) hyperplasia of dictyosomes, expansion of the zone of their location, constrictions in mitochondria, a partial enlightenment of the mitochondrial matrix at the new moon; b) the Golgi complex in the folds of karyolemma, mitochondria with the signs of degradation at the full moon. Gc, Golgi complex; Mt, mitochondria; Ns, nucleolus.
Fig. 8. Photos of the vesicles with osmiophilic granules in pinealocytes (arrows) within the deep zone of the pineal gland in mice: a) in the new moon; b) in the full moon. Semi-fine sections. Azure II stain.
We have no evidence that changes in the submandibular salivary gland are caused by the primary changes in pinealocytes, but we believe that this is the case. Apparently, it is the gravitational influence of the Earth’s natural satellite that exerts an influence (without affecting the redistribution of cells in the tissues of the pineal gland) that is sufficient to induce morphological (and probably functional) changes in pinealocytes. These changes correlate with the phases of the moon. Since redistribution of the vesicles containing osmiophilic electron-dense inclusions, i.e. peculiar micrograins, and other morphological changes are in accordance with the phases of the moon, one can deduce that the cause of the observed changes is also based on the lunar influence. This is consistent with the prediction of the lunasensor hypothesis about the role of the pineal gland as a peculiar gravisensor [9, 10].
The ultrastructure of cytoplasmic vesicles with osmiophilic inclusions is variable in pinealocytes, but very characteristic. Vesicles are separated from cytosol by a single membrane and rich in an electron-transparent component, which probably contains water-soluble hormonal factors. As for electron-dense particles in murine pinealocytes, they are known as calcified material. Similar vesicular electron-dense concretions in rats contain considerably more osmiophilic material than in mice [6, 14, 15]. Taking into account the observed increase in the number of vesicles with osmiophilic inclusions occurring at the new moon (and at the background of hyperplasia of dictyosomes and an expanding area of the Golgi complex), one can speculate that the vesicles carrying dense inclusions are formed owing to the Golgi complex of pinealocytes. It is generally accepted that the functional state of the pineal endocrine cells – light pinealocytes (LP) and dark pinealocytes (DP) – is different [1, 2, 16]. The observed lunar phase-dependent morphological changes can be a reflection of the shifts in the functional state of the pineal gland. Under the influence of recurrent lunaphasic secretion of the pineal gland, different systems and organs of the body could also periodically change some of their properties, including submandibular gland as an example of such organs. Morphological criteria for evaluating the functional status of both the pineal gland and submandibular salivary glands are well known [1, 2, 6, 7, 13, 17-20], and they therefore can be used in search of the supposed primary processes responsible for the lunaphasic sensory system of a body. In the present study, the lunaphasic morphological changes were also observed, in addition to the pineal gland, in submandibular salivary gland. But we suppose that these salivary glands respond to the hormonal signals that were generated primarily by the pineal gland. Hormonal signals from pinealocytes could affect the hypothalamus and, through it, many other targets.
It was already reported about the moon-phase dependent activity of some functional systems of the body. According to intravital imaging data concerning the pineal gland in humans, its volume in the new moon significantly deviates from its mean value for a given age . Epidemiological data indicate that the new moon is more dangerous for myocardial infarction than the full moon . At the full moon, for example, the EEG delta activity in humans was reduced by 30% in the deeper stages of sleep, and evening melatonin secretion was also greatly suppressed . Depending on the phase of the lunar cycle, human and animal behavior can change somewhat .
Thus, we have obtained the first evidence that the intrapinealocytic electron-dense concretions, or so to say micrograins, are actually redistributed in a manner coordinated with the phases of the moon. We suppose that regular changes in the gravitational influence of the moon are perceived by the cells of the pineal gland with the help of their vesicles, which simultaneously have both electron-dense osmiophilic micrograins and bioactive substances. Osmiophilic mineral concretions, after egesting into the intercellular space of a pineal gland and aggregating into larger clusters, could form there the so-called “brain sand”, which is known in this gland already for four centuries. Micrograins, still remaining within the vesicle of a cell, could begin to consolidate under the influence of the moon’s gravitational field into gradually growing aggregate. The growing micrograins could exert the mechanical effect on the adjacent cellular structures and thereby initiate the transportation of vesicles in the direction of pinealocytic clavate processes, in order to release the vesicular content into the extracellular space. Subsequently, the released hormones could flow through capillaries and liquor to the hypothalamus. Because of that, the neuroendocrine system could regulate, in a lunaphasic manner, the activity of different organs of the body, including, for example, the murine submandibular salivary gland. As an additional effect, the liquid constituent of vesicles can form so-called false vacuoles in the intercellular space of the pineal gland. Such kind of vacuoles, though without any connection with the role of the moon, was long ago described for the gerbil pineal gland .
The basic morphological effect described here – a lunaphasic mode of redistribution of pinealocytic vesicles with electron-dense concretions (i.e. micrograins surrounded by the secretory material) – can have far reaching consequences for gerontology. If the revealed effect is really used by the endocrine-gravisensory pacemaker of the lifelong clock that participates in development, maturation, and aging, then an artificial, though harmless, shutdown of this pacemaker could become the most radical procedure to repeal aging.
The interval between the point, when the physiological maturation has been completed, and the point of completion of the life itself, has no special name. It is to this interval one can apply the term phenoptosis , understanding it somewhat anew, namely as a period of an age-dependent loss of some characters of an aged organism. Just at this time, many of them, mainly quantitative traits (strength of muscles, acuity of vision, hearing and olfactory, as well as the loss of proper hormonal balance, etc.) begin to “fade”, weakening the body literally on all fronts. The duration of such phenoptotic period varies in different species. It seems most expedient to stop the signals from the endocrine-gravitational pacemaker of a pineal gland at the beginning of this period, and this will be done as soon as such procedure will become practically attainable.
This study was supported in part by a grant from the Rostock Group (CEO A. V. Chikunov).
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